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KLH in Publications

Publications of scientific research involving KLH. Abstracts or full text articles are available by link to outside sites. If you know of a KLH-related publication that is not yet on KLH Site™, let us know.

(in order by date)

Entries in Antibody Response IgG (106)


Design and evaluation of a hypoallergenic peptide-based vaccine for Salsola kali allergy.

Tabesh S, Fanuel S, Fazlollahi MR, Yekaninejad MS, Kardar GA, Razavi SA.

Int Immunopharmacol. 2018 Nov 13;66:62-68. doi: 10.1016/j.intimp.2018.10.037. [Epub ahead of print]



The Salsola kali (S. kali) pollen is one of the most important causes of allergic rhinitis in the deserts and semi-desert areas. Immunotherapy with allergen extracts remains the only available treatment addressing the underlying mechanism of allergy. However, given the low efficacy of this method, it is necessary to find more effective and alternative therapeutic interventions using molecular biology and bioinformatics tools. In this study, a hypoallergenic vaccine was designed on the basis of B-cell epitope approach for S. kali immunotherapy.


Using the Immune Epitope Database (IEDB), a 35-mer peptide was selected and chemically conjugated to a keyhole limpet hemocyanin (KLH) molecule. Specific IgG and IgE from immunized BALB/c mice sera against the vaccine (Sal k 1-KLH), S. kali extract and the recombinant protein, rSal k 1, were measured using ELISA. Also, inhibition of IgE by mouse IgG was evaluated using an inhibitory ELISA. Finally, the IgE reactivity and T-cell reactivity of the designed vaccine were evaluated by dot blot assay and MTT assay.


Vaccination with the vaccine produced high levels of protective IgG in mice, which inhibited the binding of patients IgE to recombinant proteins. The result showed that the designed vaccine, unlike the recombinant protein and extract, did not induce T-cell lymphocytes response and also exhibited decreased IgE reactivity.


The designed vaccine can be considered as a promising candidate for therapeutic allergen-specific immunotherapy.


Keyhole limpet haemocyanin – a model antigen for human immunotoxicological studies

Ashwin Swaminathan, Robyn M Lucas, Keith Dear, and Anthony J McMichael




T-cell-dependent antibody responses in the rat: Forms and sources of keyhole limpet hemocyanin matter

Lebrec H, Hock MB, Sundsmo JS, Mytych DT, Chow H, Carlock LL, Joubert MK, Reindel J, Zhou L, Bussiere JL.

J Immunotoxicology 2013 August 20 (Epub ahead of print)



T Cell Dependent Antibody Responses in the Rat: Forms and Sources of Keyhole Limpet Hemocyanin Matter

H. Lebrec1, M. B. Hock2, J. Sundsmo3, D. Mytych2, H. Chow3, L. Carlock1, M. K. Joubert2 and J. L. Bussiere2. 1 Amgen, Seattle, WA; 2 Amgen, Thousand Oaks, CA; 3 Stellar Biotechnologies, Port Hueneme, CA.

Abstract published March 2013 online.

Poster presented March 14, 2013.

The Toxicologist
Supplement to Toxicological Sciences
An Official Journal of the Society of Toxicology
ISSN 1096-6080
Volume 132, Issue 1, Page 440
March 2013



Carbohydrate-based vaccines with a glycolipid adjuvant for breast cancer

Yen-Lin Huang, Jung-Tung Hung, Sarah K. C. Cheung, Hsin-Yu Lee, Kuo-Ching Chu, Shiou-Ting Li, Yu-Chen Lin, Chien-Tai Ren, Ting-Jen R. Cheng, Tsui-Ling Hsu, Alice L. Yu, Chung-Yi Wu, and Chi-Huey Wong

Proc Natl Acad Sci U S A. 2013 February 12; 110(7): 2517–2522.


Toward Identifying the Effects of the Specific Components of Mindfulness-Based Stress Reduction on Biologic and Emotional Outcomes Among Older Adults

Gallegos AM, Hoerger M, Talbot NL, Krasner MS, Knight JM, Moynihan JA, Duberstein PR

J Altern Complement Med. 2013 Feb 5. [Epub ahead of print]


Evaluation of primary and secondary responses to a T-cell-dependent antigen, keyhole limpet hemocyanin, in rats

Kawai R, Ito S, Aida T, Hattori H, Kimura T, Furukawa T, Mori K, Sanbuissho A, Kawada T

J Immunotoxicol. 2013 Jan;10(1):40-8. doi: 10.3109/1547691X.2012.691122. Epub 2012 Sep 7


Immunodeficiency and autoimmunity in H2-O-Deficient mice


A method for inducing antigen-specific IgG production by in vitro immunization


Oral tolerance induction in humans


Preparation, identification and application of monoclonal antibody against matrix metalloproteinases-2


Synthesis of hapten and preparation of specific polyclonal antibody with high affinity for lenalidomide, the potent drug for treatment of multiple myeloma

Darwish IA, Alzoman NZ, Abuhejail RM, El-Samani TE

Chem Cent J. 2012 Oct 26;6(1):125. [Epub ahead of print]


Immunotoxic effects of cyclophosphamide and cyclosporine in the dog.

Legrand JJ, Bouchez C, Mimouni C, N'guyen A, Bouchard J, Ameller T, Descotes J.

J Immunotoxicol. 2012 Oct 3. [Epub ahead of print]


Discovery and in Vivo Evaluation of Dual PI3Kβ/δ Inhibitors

Gonzalez-Lopez de Turiso F, Shin Y, Brown M, Cardozo M, Chen Y, Fong D, Hao X, He X, Henne K, Hu YL, Johnson MG, Kohn T, Lohman J, McBride HJ, McGee LR, Medina JC, Metz D, Miner K, Mohn D, Pattaropong V, Seganish J, Simard JL, Wannberg S, Whittington DA, Yu G, Cushing TD.

J Med Chem. 2012 Sep 13;55(17):7667-85. Epub 2012 Aug 29


A method for inducing antigen-specific IgG production by in vitro immunization


Evaluation of primary and secondary responses to a T-cell-dependent antigen, keyhole limpet hemocyanin, in rats

Kawai R, Ito S, Aida T, Hattori H, Kimura T, Furukawa T, Mori K, Sanbuissho A, Kawada T

J Immunotoxicol. 2012 Sep 7. [Epub ahead of print]


Dietary intake of resveratrol enhances the adaptive immunity of aged rats


Effects of adjuvants on the immune response of pigs after intradermal administration of antigen.

Krejci J, Nechvatalova K, Kudlackova H, Leva L, Bernardy J, Toman M, Faldyna M

Res Vet Sci. 2012 Aug 25. [Epub ahead of print]


Keyhole Limpet Hemocyanin (KLH): A Unique, Unlikely Pharmaceutical Product

H. Chow, M. Sagermann and J. Sundsmo

ImVacs 2012 Abstract

ImVacs 2012 Poster

The Immunotherapeutics and Vaccine Summit
August 13-16, 2012
Cambridge, MA USA


Modulation of anti-tumor necrosis factor alpha (TNF-α) antibody secretion in mice immunized with TNF-α kinoid.

Assier E, Semerano L, Duvallet E, Delavallée L, Bernier E, Laborie M, Grouard-Vogel G, Larcier P, Bessis N, Boissier MC

Clin Vaccine Immunol. 2012 May;19(5):699-703. doi: 10.1128/CVI.05649-11. Epub 2012 Mar 21.